A retrovirus in chinook salmon (Oncorhynchus tshawytscha) with plasmacytoid leukemia and evidence for the etiology of the disease.
نویسندگان
چکیده
A plasmacytoid leukemia (PL) has caused mortalities in chinook salmon (Oncorhynchus tshawytscha) reared in seawater netpens in western British Columbia, Canada, since 1988. Kidney or eye tissues from 11 of 13 fish from netpens with clinical PL had reverse transcriptase (RT) activity. This RT activity was associated with virus particles of retrovirus morphology and buoyant density. In a transmission experiment, PL-positive donor fish tissues also had RT activity and virus particles of retrovirus morphology and buoyant density, as did recipient fish tissues following development of the disease 6 weeks postinjection with a tissue homogenate from the donor fish. Kidney and spleen tissues from fish that developed PL following injection with an inoculum that was passed through a 0.22-micron filter, in a separate experiment (M. L. Kent and S. C. Dawe. Further evidence for a viral etiology in the plasmacytoid leukemia of chinook salmon Oncorhynchus tshawytscha. Dis. Aquat. Org., in press, 1992), also exhibited RT activity. The virus particles observed by electron-microscopic examination of tissues or sucrose fractions from PL-positive fish were enveloped and were about 110-nm diameter with a central electron-dense core. Polypeptides of about M(r) 120,000, 80,000, 42,000, 27,000, 25,000, 22,000, and 19,000 were observed when purified virus particles were examined by polyacrylamide gel electrophoresis analysis. Many infectious neoplasms of animals, including fishes, are caused by retroviruses. The evidence in this study shows the presence of a retrovirus in chinook salmon with PL and further suggests a retroviral etiology of the disease. We are tentatively calling this virus salmon leukemia virus.
منابع مشابه
A Retrovirus in Chinook Salmon (Oncorhynchus tshawytscha) with Plasmacytoid Leukemia and Evidence for the Etiology of the Disease 1
A plasmacytoid leukemia (PL) has caused mortalities in chinook salmon (Oncorhynchus tshawytscha) reared in seawater netpens in western British Columbia, Canada, since 1988. Kidney or eye tissues from 11 of 13 fish from netpens with clinical PL had reverse transcriptase (RT) activity. This RT activity was associated with virus particles of retrovirus morphology and buoyant density. In a transmis...
متن کاملBiochemical and histologic evidence of plasmacytoid leukemia and salmon leukemia virus (SLV) in wild-caught chinook salmon Oncorhynchus tshawytscha from British Columbia expressing plasmacytoid leukemia
Seven of 118 wild-caught chinook salmon Oncorhynchus tshawytscha had histologic signs of plasmacytoid leukemia (PL). A pooled tissue sample from 2 of these PLpositive f ~ s h was examined and found to be Infected with a retrovirus as ~ndicated by the elevated reverse transcnptase activity associated with a density of 1.17 g cm-3 in sucrose and polypeptides with identical electrophoretic mobilit...
متن کاملExperimental transmission of a plasmacytoid leukemia of chinook salmon, Oncorhynchus tshawytscha.
A plasmacytoid leukemia of chinook salmon, Oncorhynchus tshawytscha, has recently been recognized in seawater netpens in British Columbia, Canada. The disease has occurred at several sites and has caused high mortality. Plasmacytoid leukemia is characterized by a generalized invasion of visceral tissues and the orbit of the eye by plasmacytoid cells. The disease was experimentally transmitted t...
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Survival and migration dynamics of juvenile Chinook salmon (Oncorhynchus tshawytscha) in the Sacramento-San Joaquin River Delta
متن کاملFirst report of erythrocytic inclusion body syndrome (EIBS) in chinook salmon Oncorhynchus tshawytscha in Alaska, USA.
Juvenile seawater-reared chinook salmon Oncorhynchus tshawytscha at a hatchery on Baranoff Island, Alaska, USA, sustained chronic mortality associated with anemia and mixed infections by various obligate and opportunistic pathogens including a cytoplasmic erythrocytic virus resembling the agent of erythrocytic inclusion body syndrome (EIBS). This is the first case report of EIBS in Alaska and i...
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ورودعنوان ژورنال:
- Cancer research
دوره 52 23 شماره
صفحات -
تاریخ انتشار 1992